https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41140 3O₄ nanoparticles (NP) received great attention due to their effective peroxidase like activity. The pH dependent peroxidase activity of Fe₃O₄ NP results in generation of radical ^·OH radical via the unique Fenton chemistry of iron. However, their pH dependent activity is restricted to acidic environment and dramatic loss in antibacterial activity is observed at near neutral pH. Here we describe a novel strategy to overcome the pH lacunae of citrate coated Fe₃O₄ NP by utilizing adenosine triphosphate disodium salt (ATP) as a synergistic agent to accelerate the radical ^·OH radical production and restore its antibacterial activity over a wide range of pH. This synergistic combination (30 µg/mL Fe₃O₄ NP and 2.5 mM ATP) shows a high bactericidal activity against both gram positive (B. subtilis) and gram negative (E. coli) bacterial strains, in presence of H₂O₂, at neutral pH. The synergistic effect (Fe₃O₄ NP + ATP) is determined from the viability assessment and membrane damage studies and is further confirmed by comparing the concentration of generated radical ^·OH radicals. Over all, this study illustrates ATP assisted and radical dotOH-mediated bactericidal activity of Fe₃O₄ nanozyme at near neutral pH.]]> Wed 27 Jul 2022 14:33:27 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43950 Wed 05 Oct 2022 13:05:30 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:46495 Thu 24 Nov 2022 12:01:03 AEDT ]]>